Journal: Frontiers in Cellular Neuroscience
Article Title: Modulation of Cellular Circadian Rhythms by Secondary Metabolites of Lichens
doi: 10.3389/fncel.2022.907308
Figure Lengend Snippet: Characterization of circadian luminescence rhythms in N44, U2OS, and MEF cells. (A) Experimental setup; for more details, see text. (B) Representative raw and (C) representative normalized data and sine curve fittings of Bmal1:luc (BL) and Per2:luc (P2L) luminescence recordings of hypothalamus-derived mouse N44 (N44-BL), human bone osteosarcoma epithelial (U2OS-BL), and mouse embryonic fibroblast (MEF-P2L) cells after synchronization with dexamethasone (Dex). (D–F) Luminescence period lengths (D) , rhythm phases (E) , and dampening rates (F) of the same data set. Single measures are shown; error bars indicate means ± SEM ( * p < 0.05, ** p < 0.01, *** p < 0.001; 1-way ANOVA with Dunnett’s post- test; n = 6). ns, non-significant.
Article Snippet: An embryonic mouse hypothalamic cell line (N44) was isolated and immortalized from mouse embryonic (days 15–18 post coitum ) hypothalamic primary cultures by retroviral transfer of SV40-T antigen (Cedarlane, Burlington, Canada).
Techniques: Derivative Assay